Anti-Phospho-TFRC (Ser24) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TFRC (Ser24). Anti-Phospho-TFRC (Ser24) Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse)

Blank control (blue line): HL60 (fixed with 70% ethanol Overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-phospho-Transferrin Receptor (Ser24) antibody (TMAB-11372), Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg /test.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthesised phosphopeptide: human Transferrin Receptor around the phosphorylation site of Ser24

Human

TFRC

Transferrin receptor protein 1

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferring for an overlapping C-terminal binding site.