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Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738)

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Catalog No. TMAY-01658
Alias STAT6C, STAT6B, STAT-6, IL-4-STAT, HIES6, D12S1644

Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738) is a Rabbit antibody targeting Phospho-Stat6 (Tyr641). Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738) can be used in WB.

Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738)

Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738)

😃Good
Catalog No. TMAY-01658Alias STAT6C, STAT6B, STAT-6, IL-4-STAT, HIES6, D12S1644
Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738) is a Rabbit antibody targeting Phospho-Stat6 (Tyr641). Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738) can be used in WB.
Pack SizePriceAvailabilityQuantity
20 μL$80 7-10 days
100 μL$233 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738) is a Rabbit antibody targeting Phospho-Stat6 (Tyr641). Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738) can be used in WB.
AliasSTAT6C, STAT6B, STAT-6, IL-4-STAT, HIES6, D12S1644
Ig Type
Monoclonal Rabbit IgG
Clone
6Y738
Reactivity
Human;
Species predicted to react based on 100% sequence homology: Cynomolgus, Bovin, Pig, Chicken
Verified Activity
1. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with IL4 (100 ng/mL, 15min; +), using Phospho-Stat6 (Tyr641) Antibody, Rabbit MAb at 1:10000 dilution (upper) or Anti-STAT6 Antibody, Rabbit PAb at 1:1000 dilution (lower).

2. Western blot analysis of extracts from serum-starved Hela, treated with IL4 (100 ng/mL, 15 min), using Phospho-Stat6 (Tyr641) Antibody, Rabbit MAb and other brands’ antibodies (Company C) at dilution of 1:1000, 1:10000 and 1:200000.

3. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with IL4 using Phospho-Stat6 (Tyr641) Antibody, Rabbit MAb at 1:10000 dilution. (Validation Experiment)

4. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with IL4 or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Stat6 (Tyr641) Antibody, Rabbit MAb at 1:10000 dilution. (Validation Experiment)
Application
WB
Recommended Dose
WB: 1:2000-1:20000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Tyr641 of the Human Stat6.
PurificationProtein A
AppearanceLiquid
Formulation10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5
Research BackgroundSignal transducer and activator of transcription 6 (STAT6) is a transcription factor that is activated by interleukin-4 (IL-4)-induced tyrosine phosphorylation and mediates most of the IL-4-induced gene expression. STAT6 plays a central role in exerting interleukin-4 (IL-4) mediated biological responses and is found to induce the expression of BCL2L1/BCL-XL, which is responsible for the anti-apoptotic activity of IL4. Transcriptional activation by STAT6 requires the interaction with coactivators like p300 and the CREB-binding protein (CBP). NF-κB and tyrosine-phosphorylated Stat6 can directly bind each other in vitro and in vivo, which suggests that the direct interaction between Stat6 and NF-κB may provide a basis for synergistic activation of transcription by IL-4 and activators of NF-κB.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic phosphopeptide corresponding to residues around Tyr641 of the Human Stat6
Antigen Species
Human
Biology Area
Apoptosis Transcription Factors and Regulators
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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