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Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) is a Rabbit antibody targeting Phospho-Stat5 (Tyr694). Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) can be used in IHC-P,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 20 μL | $82 | 7-10 days | 7-10 days | |
| 100 μL | $235 | 7-10 days | 7-10 days |
| Description | Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) is a Rabbit antibody targeting Phospho-Stat5 (Tyr694). Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) can be used in IHC-P,WB. |
| Synonyms | STAT5A, Stat5 (p-Y694), Stat5 (p-Tyr694), STAT-5, STAT5, p-Stat5 (Y694), p-Stat5 (Tyr694), Phospho-Stat5 (Y694), Mgf |
| Ig Type | Rabbit IgG |
| Clone | 2E826 |
| Reactivity | Human;
Predicted to React with:Human Phospho-Stat5A (Tyr694) and Human Phospho-Stat5B (Tyr699)Predicted to React with: Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus, Bovine, Pig, Sheep |
| Specificity | Reacts with: Human
Human Phospho-Stat5A (Tyr694) and Human Phospho-Stat5B (Tyr699) Predicted to React with: Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus, Bovine, Pig, Sheep |
| Verified Activity | 1. Western blot analysis of extracts from serum-starved TF-1, untreated (-) or treated with GM-CSF Antibody, Rabbit MAb at 1:1000 dilution (upper) or Anti-STAT5a Antibody, Rabbit PAb, Antigen Affinity Purified at 1:500 dilution (lower).
2. Western blot analysis of extracts from serum-starved TF-1, untreated (line A); treated with GM-CSF (line B); treated with GM-CSF and λ-phosphatase (line C) using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb at 1:1000 dilution. 3. Western blot analysis of extracts from serum-starved TF-1, untreated (line A); treated with GM-CSF or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb at 1:1000 dilution. 4. Immunohistochemical analysis of paraffin-embedded human lung cancer, using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb at 1:50 dilution. 5. Immunohistochemical analysis of paraffin-embedded human breast cancer, untreated (left) or lambda phosphatase-treated (right), using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb at 1:50 dilution. |
| Application | |
| Recommended Dose | WB: 1:1000-1:10000; IHC-P: 1:50-1:200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Tyr694 of human Phospho-Stat5. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Research Background | Signal transducers and activators of transcription 5 (STAT5) has been shown to be involved in a variety of cellular processes, including survival, proliferation, invasion, angiogenesis and immune evasion and is frequently overexpressed in human solid tumors and blood malignancies. STAT5a/b species are well known as transcription factors that regulate nuclear gene expression. Using enucleated cytoplasts for a nongenomic mechanism(s) underlying the cystic change in ER structure elicited by STAT5a/b knockdown. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: residues around Tyr694 of human Phospho-Stat5. |
| Antigen Species | Human |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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