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Anti-Phospho-Stat5 (Tyr694) Antibody (2E826)

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Catalog No. TMAY-01657
Alias STAT5A, STAT-5, STAT5, Mgf

Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) is a Rabbit antibody targeting Phospho-Stat5 (Tyr694). Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) can be used in WB,IHC-P.

Anti-Phospho-Stat5 (Tyr694) Antibody (2E826)

Anti-Phospho-Stat5 (Tyr694) Antibody (2E826)

😃Good
Catalog No. TMAY-01657Alias STAT5A, STAT-5, STAT5, Mgf
Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) is a Rabbit antibody targeting Phospho-Stat5 (Tyr694). Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) can be used in WB,IHC-P.
Pack SizePriceAvailabilityQuantity
20 μL$82 7-10 days
100 μL$235 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) is a Rabbit antibody targeting Phospho-Stat5 (Tyr694). Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) can be used in WB,IHC-P.
AliasSTAT5A, STAT-5, STAT5, Mgf
Ig Type
Monoclonal Rabbit IgG
Clone
2E826
Reactivity
Human;
Predicted to React with:Human Phospho-Stat5A (Tyr694) and Human Phospho-Stat5B (Tyr699)Predicted to React with: Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus, Bovine, Pig, Sheep
Verified Activity
1. Western blot analysis of extracts from serum-starved TF-1, untreated (-) or treated with GM-CSF Antibody, Rabbit MAb at 1:1000 dilution (upper) or Anti-STAT5a Antibody, Rabbit PAb, Antigen Affinity Purified at 1:500 dilution (lower).

2. Western blot analysis of extracts from serum-starved TF-1, untreated (line A); treated with GM-CSF (line B); treated with GM-CSF and λ-phosphatase (line C) using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb at 1:1000 dilution.

3. Western blot analysis of extracts from serum-starved TF-1, untreated (line A); treated with GM-CSF or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb at 1:1000 dilution.

4. Immunohistochemical analysis of paraffin-embedded human lung cancer, using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb at 1:50 dilution.

5. Immunohistochemical analysis of paraffin-embedded human breast cancer, untreated (left) or lambda phosphatase-treated (right), using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb at 1:50 dilution.
Application
WB,IHC-P
Recommended Dose
WB: 1:1000-1:10000; IHC-P: 1:50-1:200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Tyr694 of human Phospho-Stat5.
PurificationProtein A
AppearanceLiquid
Formulation10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5
Research BackgroundSignal transducers and activators of transcription 5 (STAT5) has been shown to be involved in a variety of cellular processes, including survival, proliferation, invasion, angiogenesis and immune evasion and is frequently overexpressed in human solid tumors and blood malignancies. STAT5a/b species are well known as transcription factors that regulate nuclear gene expression. Using enucleated cytoplasts for a nongenomic mechanism(s) underlying the cystic change in ER structure elicited by STAT5a/b knockdown.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic phosphopeptide corresponding to residues around Tyr694 of human Phospho-Stat5.
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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