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Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) is a Rabbit antibody targeting Phospho-Stat5 (Tyr694). Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) can be used in WB,IHC-P.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $82 | 7-10 days | |
100 μL | $235 | 7-10 days |
Description | Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) is a Rabbit antibody targeting Phospho-Stat5 (Tyr694). Anti-Phospho-Stat5 (Tyr694) Antibody (2E826) can be used in WB,IHC-P. |
Alias | STAT5A, STAT-5, STAT5, Mgf |
Ig Type | Monoclonal Rabbit IgG |
Clone | 2E826 |
Reactivity | Human;
Predicted to React with:Human Phospho-Stat5A (Tyr694) and Human Phospho-Stat5B (Tyr699)Predicted to React with: Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus, Bovine, Pig, Sheep |
Verified Activity | 1. Western blot analysis of extracts from serum-starved TF-1, untreated (-) or treated with GM-CSF Antibody, Rabbit MAb at 1:1000 dilution (upper) or Anti-STAT5a Antibody, Rabbit PAb, Antigen Affinity Purified at 1:500 dilution (lower). 2. Western blot analysis of extracts from serum-starved TF-1, untreated (line A); treated with GM-CSF (line B); treated with GM-CSF and λ-phosphatase (line C) using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb at 1:1000 dilution. 3. Western blot analysis of extracts from serum-starved TF-1, untreated (line A); treated with GM-CSF or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb at 1:1000 dilution. 4. Immunohistochemical analysis of paraffin-embedded human lung cancer, using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb at 1:50 dilution. 5. Immunohistochemical analysis of paraffin-embedded human breast cancer, untreated (left) or lambda phosphatase-treated (right), using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb at 1:50 dilution. |
Application | WB,IHC-P |
Recommended Dose | WB: 1:1000-1:10000; IHC-P: 1:50-1:200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Tyr694 of human Phospho-Stat5. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
Research Background | Signal transducers and activators of transcription 5 (STAT5) has been shown to be involved in a variety of cellular processes, including survival, proliferation, invasion, angiogenesis and immune evasion and is frequently overexpressed in human solid tumors and blood malignancies. STAT5a/b species are well known as transcription factors that regulate nuclear gene expression. Using enucleated cytoplasts for a nongenomic mechanism(s) underlying the cystic change in ER structure elicited by STAT5a/b knockdown. |
Conjucates | Unconjugated |
Immunogen | A synthetic phosphopeptide corresponding to residues around Tyr694 of human Phospho-Stat5. |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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