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Anti-Phospho-Src (Tyr529) Polyclonal Antibody

(Synonyms: Src (p-Y529), Src (p-Tyr529), p-Src (Y529), p-Src (Tyr529)) Copy Product Info
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Synonyms: Src (p-Y529), Src (p-Tyr529), p-Src (Y529), p-Src (Tyr529)

Catalog No. TMAC-03848 Copy Product Info
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Anti-Phospho-Src (Tyr529) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Src (Tyr529). Anti-Phospho-Src (Tyr529) Polyclonal Antibody can be used in IF,IHC,WB.

Anti-Phospho-Src
(Tyr529) Polyclonal Antibody

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2177-10 days7-10 days
100 µL$3187-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-Src (Tyr529) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Src (Tyr529). Anti-Phospho-Src (Tyr529) Polyclonal Antibody can be used in IF,IHC,WB.
SynonymsSrc (p-Y529), Src (p-Tyr529), p-Src (Y529), p-Src (Tyr529)
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of extracts from HT29 cells untreated or treated with PMA using Src (Phospho-Tyr529) Antibody TMAC-03848.
2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (Phospho-Tyr529) Antibody TMAC-03848 (left) or the same antibody preincubated with blocking peptide (right).
3. Immunofluorescence staining of methanol-fixed Hela cells using Src (Phospho-Tyr529) Antibody TMAC-03848.
4. Western blot analysis of extracts from HepG2 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using Src (Phospho-Tyr529) Antibody TMAC-03848.
Anti-Phospho-Src (Tyr529) Polyclonal Antibody,Anti-Phospho-Src (Tyr529) Polyclonal Antibody,Anti-Phospho-Src (Tyr529) Polyclonal Antibody,Anti-Phospho-Src (Tyr529) Polyclonal Antibody
Application
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
AppearanceLiquid
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research Backgroundproto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Phospho
Antigen Details
Immunogen
Peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y(p)-Q-P) derived from Human Src
Antigen Species
Human
Uniprot ID
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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