Anti-Phospho-SPHK2 (Thr614) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-SPHK2 (Thr614). Anti-Phospho-SPHK2 (Thr614) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Sheep)

1. Sample:
Cerebrum (Mouse) Lysate at 40 μg
SihA (huamn) Cell Lysate at 40 μg
Primary: Anti-p-SPHK2 (Thr614) (TMAB-11314) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kD
Observed band size: 75 kD
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-SPHK2 (Thr614)) Polyclonal Antibody, Unconjugated (TMAB-11314) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human SPHK2 around the phosphorylation site of Thr614

Human

SPHK2

Sphingosine kinase 2

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.