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Anti-Phospho-SMAD7 (Ser249) Polyclonal Antibody
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Catalog No. TMAB-01502
Alias SMAD7 (p-Ser249), SMAD7 (p-S249), SMAD7, SMAD, mothers against DPP homolog 7(Drosophila), SMAD, mothers against DPP homolog 7, SMAD family member 7, SMAD 7, SMAD, SMA-AND MAD-RELATED PROTEIN 7, p-SMAD7 (Ser249), p-SMAD7 (S249), Mothers against DPP homolog 8, Mothers against DPP homolog 7, Mothers against decapentaplegic homolog 8, Mothers against decapentaplegic homolog 7, Mothers Against Decapentaplegic Drosophila Homolog of 7, MADH8, MADH 8, MADH 7, MAD(mothers against decapentaplegic Drosophila) homolog 7, MAD mothers against decapentaplegic homolog 7, Mad homolog 7, MAD
Anti-Phospho-SMAD7 (Ser249) Polyclonal Antibody is a Rabbit antibody targeting Phospho-SMAD7 (Ser249). Anti-Phospho-SMAD7 (Ser249) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-SMAD7 (Ser249) Polyclonal Antibody
Anti-Phospho-SMAD7 (Ser249) Polyclonal Antibody
😃Good
Catalog No. TMAB-01502Alias SMAD7 (p-Ser249), SMAD7 (p-S249), SMAD7, SMAD, mothers against DPP homolog 7(Drosophila), SMAD, mothers against DPP homolog 7, SMAD family member 7, SMAD 7, SMAD, SMA-AND MAD-RELATED PROTEIN 7, p-SMAD7 (Ser249), p-SMAD7 (S249), Mothers against DPP homolog 8, Mothers against DPP homolog 7, Mothers against decapentaplegic homolog 8, Mothers against decapentaplegic homolog 7, Mothers Against Decapentaplegic Drosophila Homolog of 7, MADH8, MADH 8, MADH 7, MAD(mothers against decapentaplegic Drosophila) homolog 7, MAD mothers against decapentaplegic homolog 7, Mad homolog 7, MAD
Anti-Phospho-SMAD7 (Ser249) Polyclonal Antibody is a Rabbit antibody targeting Phospho-SMAD7 (Ser249). Anti-Phospho-SMAD7 (Ser249) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-SMAD7 (Ser249) Polyclonal Antibody is a Rabbit antibody targeting Phospho-SMAD7 (Ser249). Anti-Phospho-SMAD7 (Ser249) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | SMAD7 (p-Ser249), SMAD7 (p-S249), SMAD7, SMAD, mothers against DPP homolog 7(Drosophila), SMAD, mothers against DPP homolog 7, SMAD family member 7, SMAD 7, SMAD, SMA-AND MAD-RELATED PROTEIN 7, p-SMAD7 (Ser249), p-SMAD7 (S249), Mothers against DPP homolog 8, Mothers against DPP homolog 7, Mothers against decapentaplegic homolog 8, Mothers against decapentaplegic homolog 7, Mothers Against Decapentaplegic Drosophila Homolog of 7, MADH8, MADH 8, MADH 7, MAD(mothers against decapentaplegic Drosophila) homolog 7, MAD mothers against decapentaplegic homolog 7, Mad homolog 7, MAD |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Pig,Cow,GuineaPig) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-SMAD7 (Ser249)) Polyclonal Antibody, Unconjugated (TMAB-01502) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-SMAD7 (Ser249)) Polyclonal Antibody, Unconjugated (TMAB-01502) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-SMAD7 (Ser249)) Polyclonal Antibody, Unconjugated (TMAB-01502) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-SMAD7 (Ser249)) Polyclonal Antibody, Unconjugated (TMAB-01502) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Primary: Anti-Phospho-SMAD7 (Ser249) (TMAB-01502) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 50 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm. Note=Interaction with NEDD4L or RNF111 or induces translocation from the nucleus to the cytoplasm. TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Immunogen | KLH conjugated synthesised phosphopeptide: human SMAD7 around the phosphorylation site of Ser249 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | SMADs,SMADs |
| Molecular Weight | Theoretical: 46 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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