Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-Phospho-SMAD5 (Ser463, 465) Antibody (2F385)
😃Good
Catalog No. TMAC-03270
Alias SMAD5 (p-Ser463, 465), SMAD5 (p-S463, 465), SMAD family member 5, SMAD 5, SMAD, SMA and MAD related protein 5, p-SMAD5 (Ser463, 465), p-SMAD5 (S463, 465), Phospho-SMAD5 (S463, 465), MusMLP, mothers against DPP homolog 5, Mothers against DPP homolog 5, mothers against decapentaplegic homolog 5, MADH5, MADH 5, MAD homolog 5, MAD, JV5 1, hSmad5, Dwfc, DKFZp781O1323, DKFZp781C1895
Anti-Phospho-SMAD5 (S463, 465) Antibody (2F385) is a Rabbit antibody targeting Phospho-SMAD5 (S463, 465). Anti-Phospho-SMAD5 (S463, 465) Antibody (2F385) can be used in WB,ICC,IHC.
Anti-Phospho-SMAD5 (Ser463, 465) Antibody (2F385)
Anti-Phospho-SMAD5 (Ser463, 465) Antibody (2F385)
😃Good
Catalog No. TMAC-03270Alias SMAD5 (p-Ser463, 465), SMAD5 (p-S463, 465), SMAD family member 5, SMAD 5, SMAD, SMA and MAD related protein 5, p-SMAD5 (Ser463, 465), p-SMAD5 (S463, 465), Phospho-SMAD5 (S463, 465), MusMLP, mothers against DPP homolog 5, Mothers against DPP homolog 5, mothers against decapentaplegic homolog 5, MADH5, MADH 5, MAD homolog 5, MAD, JV5 1, hSmad5, Dwfc, DKFZp781O1323, DKFZp781C1895
Anti-Phospho-SMAD5 (S463, 465) Antibody (2F385) is a Rabbit antibody targeting Phospho-SMAD5 (S463, 465). Anti-Phospho-SMAD5 (S463, 465) Antibody (2F385) can be used in WB,ICC,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
Add to Cart
Add to Quotation
Bulk & Custom
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Questions
View MoreResource Download
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Phospho-SMAD5 (Ser463, 465) Antibody (2F385) is a Rabbit antibody targeting Phospho-SMAD5 (Ser463, 465). Anti-Phospho-SMAD5 (Ser463, 465) Antibody (2F385) can be used in WB,ICC,IHC. |
| Synonyms | SMAD5 (p-Ser463, 465), SMAD5 (p-S463, 465), SMAD family member 5, SMAD 5, SMAD, SMA and MAD related protein 5, p-SMAD5 (Ser463, 465), p-SMAD5 (S463, 465), Phospho-SMAD5 (S463, 465), MusMLP, mothers against DPP homolog 5, Mothers against DPP homolog 5, mothers against decapentaplegic homolog 5, MADH5, MADH 5, MAD homolog 5, MAD, JV5 1, hSmad5, Dwfc, DKFZp781O1323, DKFZp781C1895 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Phospho-Smad5 (S463/465) on different lysates using anti-Phospho-Smad5 (S463/465) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain, Lane 2: Rat brain. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin. 7. ICC staining Phospho-Smad5 (S463/465) in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Phospho-Smad5 (S463/465) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | A synthesized phosphopeptide: human Smad5 around the phosphorylation site of Ser463 and 465 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 58 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Sci Citations
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
Preview
Related Tags: buy Anti-Phospho-SMAD5 (Ser463, 465) Antibody (2F385) | purchase Anti-Phospho-SMAD5 (Ser463, 465) Antibody (2F385) | Anti-Phospho-SMAD5 (Ser463, 465) Antibody (2F385) cost | order Anti-Phospho-SMAD5 (Ser463, 465) Antibody (2F385) | Anti-Phospho-SMAD5 (Ser463, 465) Antibody (2F385) molecular weight
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.