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Anti-Phospho-Smad3 (Ser423+Ser425) Antibody (8S428)
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Catalog No. TMAB-11301
Anti-Phospho-Smad3 (Ser423+Ser425) Antibody (8S428) is a Rabbit antibody targeting Phospho-Smad3 (Ser423+Ser425). Anti-Phospho-Smad3 (Ser423+Ser425) Antibody (8S428) can be used in WB.
Anti-Phospho-Smad3 (Ser423+Ser425) Antibody (8S428)
Anti-Phospho-Smad3 (Ser423+Ser425) Antibody (8S428)
😃Good
Catalog No. TMAB-11301
Anti-Phospho-Smad3 (Ser423+Ser425) Antibody (8S428) is a Rabbit antibody targeting Phospho-Smad3 (Ser423+Ser425). Anti-Phospho-Smad3 (Ser423+Ser425) Antibody (8S428) can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-Smad3 (Ser423+Ser425) Antibody (8S428) is a Rabbit antibody targeting Phospho-Smad3 (Ser423+Ser425). Anti-Phospho-Smad3 (Ser423+Ser425) Antibody (8S428) can be used in WB. |
| Ig Type | IgG |
| Clone | 8S428 |
| Reactivity | Human (predicted:Mouse) |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane. MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human Smad3 around the phosphorylation site of Ser423/425 |
| Antigen Species | Human |
| Gene Name | SMAD3 |
| Gene ID | |
| Protein Name | Mothers against decapentaplegic homolog 3 |
| Uniprot ID | |
| Function | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Molecular Weight | Theoretical: 47 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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