Anti-Phospho-Smad3 (Ser423+Ser425) Antibody (8S428) is a Rabbit antibody targeting Phospho-Smad3 (Ser423+Ser425). Anti-Phospho-Smad3 (Ser423+Ser425) Antibody (8S428) can be used in WB.

IgG

8S428

Human (predicted:Mouse)

A549 (H) cells were treated with or without hTGF-β (10 ng/ml) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-Smad3 (Ser423+Ser425) monoclonal antibody, unconjugated (TMAB-11301) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000

Monoclonal

KLH conjugated Synthesised phosphopeptide: human Smad3 around the phosphorylation site of Ser423/425

Human

SMAD3

Mothers against decapentaplegic homolog 3

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.