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Anti-Phospho-SMAD2 (Ser465, 467) Antibody (3X677)
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Catalog No. TMAY-01523
Alias SMAD2, MADR2, MADH2, LDS6, JV18-1, JV18, hSMAD2, hMAD-2, CHTD8
Anti-Phospho-SMAD2 (Ser465, 467) Antibody (3X677) is a Rabbit antibody targeting Phospho-SMAD2 (Ser465, 467). Anti-Phospho-SMAD2 (Ser465, 467) Antibody (3X677) can be used in WB.
Anti-Phospho-SMAD2 (Ser465, 467) Antibody (3X677)
Anti-Phospho-SMAD2 (Ser465, 467) Antibody (3X677)
😃Good
Catalog No. TMAY-01523Alias SMAD2, MADR2, MADH2, LDS6, JV18-1, JV18, hSMAD2, hMAD-2, CHTD8
Anti-Phospho-SMAD2 (Ser465, 467) Antibody (3X677) is a Rabbit antibody targeting Phospho-SMAD2 (Ser465, 467). Anti-Phospho-SMAD2 (Ser465, 467) Antibody (3X677) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $82 | 7-10 days | |
| 100 μL | $234 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-Phospho-SMAD2 (Ser465, 467) Antibody (3X677) is a Rabbit antibody targeting Phospho-SMAD2 (Ser465, 467). Anti-Phospho-SMAD2 (Ser465, 467) Antibody (3X677) can be used in WB. |
| Alias | SMAD2, MADR2, MADH2, LDS6, JV18-1, JV18, hSMAD2, hMAD-2, CHTD8 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 3X677 |
| Reactivity | Human;
Predicted to React with:Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus, Bovine, Zebrafish |
| Verified Activity | 1. Western blot analysis of extracts from serum-starved Hela, untreated(line A) or treated with TGF beta 1 (5 ng/mL, 120min; +)(line B), using Phospho-SMAD2 (Ser465, 467) rabbit monoclonal Antibody at 1:1000 dilution (upper) or Anti-SMAD2 Antibody, Rabbit polyclonal at 1:100000 dilution (lower). 2. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with TGFβ1 (5 ng/mL, 120 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-SMAD2 (Ser465, 467) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) 3. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with TGFβ1 (5 ng/mL, 120min; +); treated with TGFβ1 and λ-phosphatase (line C) using Phospho-SMAD2 (Ser465, 467) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) |
| Application | WB |
| Recommended Dose | WB: 1:500-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the residues around (Ser465, 467) of Human Phospho-SMAD2. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Research Background | SMAD2 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. Phosphorylated SMAD2 is able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide corresponding to the residues around (Ser465, 467) of Human Phospho-SMAD2 |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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