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Anti-Phospho-Smad2 (Ser245+Ser250+Ser255) Polyclonal Antibody
🥰Excellent
Catalog No. TMAB-11292
Antibody Type: Rabbit Polyclonal
Application: WB,IHC-P,IHC-Fr,IF,FCM
Reactivity: Human,Mouse (predicted:Rat,Dog,Pig,Cow)
Anti-Phospho-Smad2 (Ser245+Ser250+Ser255) Polyclonal Antibody
Anti-Phospho-Smad2 (Ser245+Ser250+Ser255) Polyclonal Antibody
🥰Excellent
Catalog No. TMAB-11292
Antibody Type: Rabbit Polyclonal
Application: WB,IHC-P,IHC-Fr,IF,FCM
Reactivity: Human,Mouse (predicted:Rat,Dog,Pig,Cow)
Application: WB,IHC-P,IHC-Fr,IF,FCM
Reactivity: Human,Mouse (predicted:Rat,Dog,Pig,Cow)
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-Smad2 (Ser245+Ser250+Ser255) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Smad2 (Ser245+Ser250+Ser255). Anti-Phospho-Smad2 (Ser245+Ser250+Ser255) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow) |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. |
| Tissue Specificity | Expressed at high levels in skeletal muscle, heart and placenta. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012] |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human Smad2 around the phosphorylation site of Ser245/250/255 |
| Antigen Species | Human |
| Gene Name | SMAD2 |
| Gene ID | |
| Protein Name | Mothers against decapentaplegic homolog 2 |
| Uniprot ID | |
| Function | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Molecular Weight | Theoretical: 58 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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