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Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody
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Catalog No. TMAB-01494
Alias Transforming growth factor-beta-signaling protein 1, Transforming growth factor beta signaling protein 1, TGF beta signaling protein 1, Spinal muscular atrophy distal with upper limb predominance, Smad1/5 (p-Ser463, 465), Smad1/5 (p-S463, 465), SMAD-1, Smad1, SMAD1, SMAD mothers against DPP homolog 1 Drosophila, SMAD family member 1, Sma and Mad related protein 1, p-Smad1/5 (Ser463, 465), p-Smad1/5 (S463, 465), Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, Mad-related protein 1, MADR1, Madr 1, MADH1, MADH 1, Mad related protein 1, MAD mothers against decapentaplegic homolog 1, MAD homolog 1, JV41, JV4 1, JV 41, hSMAD1, hSMAD 1, DwfA, Dwarfin A, BSP1, BSP 1
Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Smad1/5 (Ser463, 465). Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody
Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody
😃Good
Catalog No. TMAB-01494Alias Transforming growth factor-beta-signaling protein 1, Transforming growth factor beta signaling protein 1, TGF beta signaling protein 1, Spinal muscular atrophy distal with upper limb predominance, Smad1/5 (p-Ser463, 465), Smad1/5 (p-S463, 465), SMAD-1, Smad1, SMAD1, SMAD mothers against DPP homolog 1 Drosophila, SMAD family member 1, Sma and Mad related protein 1, p-Smad1/5 (Ser463, 465), p-Smad1/5 (S463, 465), Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, Mad-related protein 1, MADR1, Madr 1, MADH1, MADH 1, Mad related protein 1, MAD mothers against decapentaplegic homolog 1, MAD homolog 1, JV41, JV4 1, JV 41, hSMAD1, hSMAD 1, DwfA, Dwarfin A, BSP1, BSP 1
Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Smad1/5 (Ser463, 465). Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Smad1/5 (Ser463, 465). Anti-Phospho-Smad1/5 (Ser463, 465) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Alias | Transforming growth factor-beta-signaling protein 1, Transforming growth factor beta signaling protein 1, TGF beta signaling protein 1, Spinal muscular atrophy distal with upper limb predominance, Smad1/5 (p-Ser463, 465), Smad1/5 (p-S463, 465), SMAD-1, Smad1, SMAD1, SMAD mothers against DPP homolog 1 Drosophila, SMAD family member 1, Sma and Mad related protein 1, p-Smad1/5 (Ser463, 465), p-Smad1/5 (S463, 465), Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, Mad-related protein 1, MADR1, Madr 1, MADH1, MADH 1, Mad related protein 1, MAD mothers against decapentaplegic homolog 1, MAD homolog 1, JV41, JV4 1, JV 41, hSMAD1, hSMAD 1, DwfA, Dwarfin A, BSP1, BSP 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat,Pig (predicted:Chicken,Dog,Cow,Horse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Smad1 5 (Ser463 + Ser465)) Polyclonal Antibody, Unconjugated (TMAB-01494) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Smad1-5 (Ser463+ Ser465)) Polyclonal Antibody, Unconjugated (TMAB-01494) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Cerebrum (Mouse) Lysate at 40 μg Stomach (Mouse) Lysate at 40 μg Primary: Anti-Phospho-Smad1/5 (Ser463 + Ser465) (TMAB-01494) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 62 kDa 4. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Heart (Mouse) Lysate at 40 μg Lane 3: Testis (Mouse) Lysate at 40 μg Lane 4: Skin (Mouse) Lysate at 40 μg Lane 5: Kidney (Mouse) Lysate at 40 μg Lane 6: Cerebrum (Rat) Lysate at 40 μg Lane 7: Testis (Rat) Lysate at 40 μg Lane 8: Skin (Rat) Lysate at 40 μg Lane 9: Kidney (Rat) Lysate at 40 μg Lane 10: Huvec (Human) Cell Lysate at 30 μg Lane 11: A549 (Human) Cell Lysate at 30 μg Lane 12: Hela (Human) Cell Lysate at 30 μg Lane 13: HT1080 (Human) Cell Lysate at 30 μg Lane 14: A431 (Human) Cell Lysate at 30 μg Primary: Anti-Phospho-Smad3 (Ser463 + Ser465) (TMAB-01494) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 54 kDa |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human Smad1/5 around the phosphorylation site of Ser463/465 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | TGF,SMADs,SMADs,Cytoplasmic |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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