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Anti-Phospho-SMAD1/5/9 (Ser463/Ser465/Ser467) Antibody (5N816)
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Catalog No. TMAB-11291 Copy Product Info
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Anti-Phospho-SMAD1/5/9 (Ser463/Ser465/Ser467) Antibody (5N816) is a Rabbit antibody targeting Phospho-SMAD1/5/9 (Ser463/Ser465/Ser467). Anti-Phospho-SMAD1/5/9 (Ser463/Ser465/Ser467) Antibody (5N816) can be used in WB,FCM.
Anti-Phospho-SMAD1/5/9
(Ser463/Ser465/Ser467) Antibody
(5N816)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 25 µL | $150 | 7-10 days | 7-10 days | |
| 50 µL | $264 | 7-10 days | 7-10 days | |
| 100 µL | $473 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-Phospho-SMAD1/5/9 (Ser463/Ser465/Ser467) Antibody (5N816) is a Rabbit antibody targeting Phospho-SMAD1/5/9 (Ser463/Ser465/Ser467). Anti-Phospho-SMAD1/5/9 (Ser463/Ser465/Ser467) Antibody (5N816) can be used in WB,FCM. |
| Ig Type | IgG |
| Clone | 5N816 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | The HepG2 (treated with Human BMP2 (50 ng/ml, 30 min)) (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-phospho-SMAD1/5/9 (S463/S465/S467) antibody (TMAB-11291, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. |
| Tissue Specificity | Ubiquitous. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. |
| Immunogen | A synthesized peptide: human SMAD1 around the phosphorylation site of S463 and S465 |
| Antigen Species | Human |
| Gene Name | SMAD5 |
| Gene ID | |
| Protein Name | Mothers against decapentaplegic homolog 5 |
| Uniprot ID | |
| Function | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). |
| Molecular Weight | Theoretical: 52 kDa. Actual: 52 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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