Anti-Phospho-SGK1 (Thr256) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-SGK1 (Thr256). Anti-Phospho-SGK1 (Thr256) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat)

1. TiTissue/cell: human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Phospho-SGK1 (Thr256) Polyclonal Antibody, Unconjugated (TMAB-11275) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: Pancreas (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-SGK1 (Thr256) (TMAB-11275) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 51 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human SGK1 around the phosphorylation site of Thr256

Human

SGK1

Serine/threonine-protein kinase Sgk1

Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1.