Anti-Phospho-SCNN1B (Thr615) Polyclonal Antibody is a Rabbit antibody targeting Phospho-SCNN1B (Thr615). Anti-Phospho-SCNN1B (Thr615) Polyclonal Antibody can be used in WB,ICC/IF,FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Cow,Horse,Rabbit,GuineaPig)

1. Sample:
Siha (Human) Cell Lysate at 40 μg
A549 (Human) Cell Lysate at 40 μg
Primary: Anti-p-SCNN1b (Thr615) (TMAB-11269) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kD
Observed band size: 73 kD
2. MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-SCNN1B (Thr615)) polyclonal Antibody, Unconjugated (TMAB-11269) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
3. Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-phospho-SCNN1B (Thr615) antibody (TMAB-11269)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; ICC/IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human SCNN1B around the phosphorylation site of Thr615

Human

SCNN1B

Epithelial sodium channel subunit beta

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.