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Anti-Phospho-S6K1/RPS6KB1 (Thr229) Polyclonal Antibody
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Catalog No. TMAC-03018
Anti-Phospho-S6K1/RPS6KB1 (Thr229) Polyclonal Antibody is a Rabbit antibody targeting Phospho-S6K1/RPS6KB1 (Thr229). Anti-Phospho-S6K1/RPS6KB1 (Thr229) Polyclonal Antibody can be used in WB.
Anti-Phospho-S6K1/RPS6KB1 (Thr229) Polyclonal Antibody
Anti-Phospho-S6K1/RPS6KB1 (Thr229) Polyclonal Antibody
😃Good
Catalog No. TMAC-03018
Anti-Phospho-S6K1/RPS6KB1 (Thr229) Polyclonal Antibody is a Rabbit antibody targeting Phospho-S6K1/RPS6KB1 (Thr229). Anti-Phospho-S6K1/RPS6KB1 (Thr229) Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $218 | 7-10 days | |
| 100 μL | $318 | 7-10 days |
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Chemical Properties
| Description | Anti-Phospho-S6K1/RPS6KB1 (Thr229) Polyclonal Antibody is a Rabbit antibody targeting Phospho-S6K1/RPS6KB1 (Thr229). Anti-Phospho-S6K1/RPS6KB1 (Thr229) Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Application | |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at 'Thr-412', which is proposed to be a negative feedback mechanism for the RPS6KB1 apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of Threonine 229 (N-H-Q(p)-G-H) derived from Human p70 S6 Kinase |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 70 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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