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Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580)
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Catalog No. TMAY-01581
Alias S6, OK/SW-cl.2, eS6
Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580) is a Rabbit antibody targeting Phospho-S6 Ribosomal Protein (Ser235, 236). Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580) can be used in WB.
Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580)
Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580)
😃Good
Catalog No. TMAY-01581Alias S6, OK/SW-cl.2, eS6
Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580) is a Rabbit antibody targeting Phospho-S6 Ribosomal Protein (Ser235, 236). Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $80 | 7-10 days | |
| 100 μL | $233 | 7-10 days |
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Product Introduction
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Chemical Properties
| Description | Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580) is a Rabbit antibody targeting Phospho-S6 Ribosomal Protein (Ser235, 236). Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580) can be used in WB. |
| Alias | S6, OK/SW-cl.2, eS6 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 1E580 |
| Reactivity | Human;
Species predicted to react based on 100% sequence homology: mouse |
| Verified Activity | 1. Western blot analysis of extracts from serum-starved Hek293, untreated (-) or treated with IGF1 (100ng/ml, 15min; +), using Phospho-S6 Ribosomal Protein (Ser235, 236) rabbit Monoclonal Antibody at 1:1000 dilution (upper) or anti-S6 Ribosomal Protein antibody (lower) at 1:1000 dilution. 2. Western blot analysis of extracts from serum-starved HEK293, treated with IGF1 (100 ng/mL, 15min), without peptide (line A) or antigen-specific phosphopeptide (line B) or antigen-specific peptide (line C) using Phospho-S6 Ribosomal (Ser235, 236) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) 3. Western blot analysis of extracts from serum-starved HEK293, treated with IGF1 (100ng/mL, 15min) (line A); treated with IGF1 and λ-phosphatase (line B) using Phospho-S6 Ribosomal (Ser235, 236) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) |
| Application | WB |
| Recommended Dose | WB: 1:1000-1:5000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to residues around (Ser235, 236) of Human Phospho-S6 Ribosomal Protein. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Research Background | Ribosomal protein S6 (rpS6), a component of the 40S ribosomal subunit, is involved in multiple cellular bioactivities. RPS6 is a downstream substrate of PI3K/Akt/mTOR/ kinase and MAPK kinase pathways and its dephosphorylation is also triggered by DNA double-strand breaks. The ribosomal protein S6 (RPS6) is a downstream component of the signaling mediated by the target of rapamycin (TOR) kinase that acts as a central regulator of the key metabolic processes, such as protein translation and ribosome biogenesis, in response to various environmental cues. mTORC1/rpS6 signaling complex promoted Sertoli blood-testis barrier (BTB) remodeling by perturbing Sertoli cell-cell adhesion site known as the basal ectoplasmic specialization (ES). mTORC1/rpS6 complex also promoted disruption of spermatid adhesion at the Sertoli-spermatid interface called the apical ES. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide corresponding to residues around (Ser235, 236) of Human Phospho-S6 Ribosomal Protein |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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