Anti-Phospho-Rb2 p130 (Thr986) Antibody (8C579) is a Rabbit antibody targeting Phospho-Rb2 p130 (Thr986). Anti-Phospho-Rb2 p130 (Thr986) Antibody (8C579) can be used in WB,IP.

IgG

8C579

Human,Mouse

Western blot analysis of (1) Jurkat cell lysate; (2) Jurkat cell treated with phosphatase lysate. Using Phospho-Rb2 p130 (T986) (TMAB-11233) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:1000-2000; IP: 1:20-50

Monoclonal

A synthesized peptide: human RBL2 around the phosphorylation site of T986

Human

RBL2

Retinoblastoma-like protein 2

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.