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Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody

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Catalog No. TMAC-03539
Alias Ras-GRF1 (p-Ser916), Ras-GRF1 (p-S916), p-Ras-GRF1 (Ser916), p-Ras-GRF1 (S916)

Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Ras-GRF1 (Ser916). Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody can be used in IHC.

Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody

Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody

😃Good
Catalog No. TMAC-03539Alias Ras-GRF1 (p-Ser916), Ras-GRF1 (p-S916), p-Ras-GRF1 (Ser916), p-Ras-GRF1 (S916)
Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Ras-GRF1 (Ser916). Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody can be used in IHC.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2177-10 days7-10 days
100 μL$3177-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Ras-GRF1 (Ser916). Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody can be used in IHC.
SynonymsRas-GRF1 (p-Ser916), Ras-GRF1 (p-S916), p-Ras-GRF1 (Ser916), p-Ras-GRF1 (S916)
Ig Type
IgG
Reactivity
Human,Mouse
Verified Activity
1. Immunohistochemical analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody TMAC-03539 (left) or the same antibody preincubated with blocking peptide (right).
verifiedActivity
Application
IHC
Recommended Dose
IHC: 1:50-100
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
AppearanceLiquid
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundRas activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Phospho
Antigen Details
Immunogen
Peptide sequence around phosphorylation site of Serine 916(R-M-S(p)-L-A) derived from Mouse Ras-GRF1
Antigen Species
mouse
Uniprot ID
P27671
Chemical Properties
Molecular WeightActual: 145 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Keywords

Ras-GRF1 (pSer916)Ras-GRF1 (pS916)Ras-GRF1 (phospho-Ser916)Ras-GRF1 (phospho-S916)pRas-GRF1 (Ser916)pRas-GRF1 (S916)
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