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Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody (Synonyms: Ras-GRF1 (p-Ser916), Ras-GRF1 (p-S916), p-Ras-GR...)
Catalog No. TMAC-03539 Copy Product Info
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Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Ras-GRF1 (Ser916). Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody can be used in IHC.
Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAC-03539
Synonyms Ras-GRF1 (p-Ser916), Ras-GRF1 (p-S916), p-Ras-GRF1 (Ser916), p-Ras-GRF1 (S916)
Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Ras-GRF1 (Ser916). Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody can be used in IHC.
Anti-Phospho-Ras-GRF1
(Ser916) Polyclonal Antibody
(Ser916) Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $217 | 7-10 days | 7-10 days | |
| 100 μL | $317 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Ras-GRF1 (Ser916). Anti-Phospho-Ras-GRF1 (Ser916) Polyclonal Antibody can be used in IHC. |
| Synonyms | Ras-GRF1 (p-Ser916), Ras-GRF1 (p-S916), p-Ras-GRF1 (Ser916), p-Ras-GRF1 (S916) |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody TMAC-03539 (left) or the same antibody preincubated with blocking peptide (right).  |
| Application | |
| Recommended Dose | IHC: 1:50-100 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of Serine 916(R-M-S(p)-L-A) derived from Mouse Ras-GRF1 |
| Antigen Species | mouse |
| Uniprot ID |
| Molecular Weight | Actual: 145 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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