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Anti-Phospho-RAF1 (Ser301) Polyclonal Antibody 2

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Catalog No. TMAC-00955

Anti-Phospho-RAF1 (Ser301) Polyclonal Antibody is a Rabbit antibody targeting Phospho-RAF1 (Ser301). Anti-Phospho-RAF1 (Ser301) Polyclonal Antibody can be used in WB.

Anti-Phospho-RAF1 (Ser301) Polyclonal Antibody 2

Anti-Phospho-RAF1 (Ser301) Polyclonal Antibody 2

😃Good
Catalog No. TMAC-00955
Anti-Phospho-RAF1 (Ser301) Polyclonal Antibody is a Rabbit antibody targeting Phospho-RAF1 (Ser301). Anti-Phospho-RAF1 (Ser301) Polyclonal Antibody can be used in WB.
Pack SizePriceAvailabilityQuantity
50 μL$2167-10 days
100 μL$3167-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-RAF1 (Ser301) Polyclonal Antibody is a Rabbit antibody targeting Phospho-RAF1 (Ser301). Anti-Phospho-RAF1 (Ser301) Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Application
Recommended Dose
WB: 1:500-3000
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
AppearanceLiquid
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundSerine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Phospho
Antigen Details
Immunogen
Peptide sequence around phosphorylation site of Serine301 (N-L-S(p)-P-T) derived from Human C-RAF
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightActual: 75 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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