Anti-Phospho-PTPN6 (Tyr536) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PTPN6 (Tyr536). Anti-Phospho-PTPN6 (Tyr536) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human (predicted:Dog,Horse)

1. Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-PTPN6 (Tyr564)) Polyclonal Antibody, Unconjugated (TMAB-11199) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
A549 Cell (Human) Lysate at 40 μg
MCF-7 Cell (Human) Lysate at 40 μg
Primary: Anti-phospho-PTPN6 (Tyr564) (TMAB-11199) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
3. Sample: A431 Cell (Human) Lysate at 40 μg
Primary: Anti-phospho-PTPN6 (Tyr564) (TMAB-11199) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
4. Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-phospho-PTPN6 (Tyr564) / AF647 Conjugated antibody (TMAB-11199-AF647)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG- AF647.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human PTPN6 around the phosphorylation site of Tyr536

Human

PTPN6

Tyrosine-protein phosphatase non-receptor type 6

Modulates signaling by tyrosine phosphorylated cellcsurface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.