Anti-Phospho-PTPN11 (Tyr511) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PTPN11 (Tyr511). Anti-Phospho-PTPN11 (Tyr511) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Chicken,Pig,Cow,Rabbit,Zebrafish,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PTPN11 (Tyr511)) Polyclonal Antibody, Unconjugated (TMAB-11194) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PTPN11 (Tyr511)) Polyclonal Antibody, Unconjugated (TMAB-11194) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Measured by its binding ability in a indirect ELISA. Immobilized Human phospho-PTPN11 protein, His Tag at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human phospho-PTPN11 Antibody, the EC50 is 10715 ng/mL.

IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human PTPN11 around the phosphorylation site of Tyr511

Human

PTPN11

Tyrosine-protein phosphatase non-receptor type 11

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.