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Anti-Phospho-PRKN (Ser65) Polyclonal Antibody
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Catalog No. TMAB-01487
Anti-Phospho-PRKN (Ser65) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKN (Ser65). Anti-Phospho-PRKN (Ser65) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-PRKN (Ser65) Polyclonal Antibody
Anti-Phospho-PRKN (Ser65) Polyclonal Antibody
😃Good
Catalog No. TMAB-01487
Anti-Phospho-PRKN (Ser65) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKN (Ser65). Anti-Phospho-PRKN (Ser65) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-PRKN (Ser65) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKN (Ser65). Anti-Phospho-PRKN (Ser65) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Pig,Cow,Horse,Sheep,Monkey) |
| Verified Activity | 1. Sample: Thymus (Mouse) Lysate at 40 μg Liver (Mouse) Lysate at 40 μg Lung (Mouse) Lysate at 40 μg Skeletal muscle (Mouse) Lysate at 40 μg Primary: Anti-phospho-Parkin (Ser65) (TMAB-01487) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa 2. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Lung (Mouse) Lysate at 40 μg Lane 3: SH-SY5Y (Human) Cell Lysate at 30 μg Lane 4: 293T (Human) Cell Lysate at 30 μg Lane 5: MCF-7 (Human) Cell Lysate at 30 μg Lane 6: Jurkat (Human) Cell Lysate at 30 μg Primary: Anti-phospho-Parkin (Ser65) (TMAB-01487) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 50 kDa 3. Sample: A673 (Human) cell Lysate at 40 μg Primary: Anti-p-Parkin (Ser65) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa 4. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Parkin (Ser65)) Polyclonal Antibody, Unconjugated (TMAB-01487) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytosol. Nucleus. Endoplasmic reticulum. Mitochondrion. Note=Mainly localizes in the cytosol. Co-localizes with SYT11 in neutrites. Co-localizes with SNCAIP in brainstem Lewy bodies. Relocates to dysfunctional mitochondria that have lost the mitochondrial membrane potential; recruitment to mitochondria is PINK1-dependent. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Parkinson's Disease, the second most common neurodegenerative disease after Alzheimer's Disease, is characterized by the loss of dopaminergic neurons and the presence of Lewy bodies (comprised of alpha synuclein and parkin inclusions). Autosomal Recessive Juvenile Parkinsonism (AR-JP) is a recently described form of Parkinson's Disease that has been linked to a gene that codes for parkin. Parkin, a 52 kDa protein, has a suggested role in the ubiquitin/proteasome pathway for protein degradation. The amino terminus bears sequence homology to ubiquitin while functionally it acts as a RING type ubiquitin protein ligase (E3) that coordinates the transfer of ubiquitin to substrate proteins, thus targeting them for degradation by the proteasome. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human Parkin around the phosphorylation site of Ser65 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Parkin / PARK,Mitophagy fission and fusion,RING Finger E3 Ligase |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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