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TOPBioactivityRelated Conjugates and FormulationsAntigen DetailsChemical infoStorage&SolubilityCalculator

Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody

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Catalog No. TMAC-03308
Alias Protein kinase C zeta, Protein kinase C gamma, Protein kinase C epsilon, Protein kinase C delta, Protein kinase C beta, Protein kinase C alpha, Protein kinase C, PRKCZ, PRKCG, PRKCE, PRKCD, PRKCB2, PRKCB1, PRKCB, PRKCA (p-Thr197), PRKCA (p-T197), PRKCA, p-PRKCA (Thr197), p-PRKCA (T197), PKCG, PKCE, PKCD, PKCB, PKC-alpha, PKC-A, PKCA, PKC2, PKC zeta, PKC gamma, PKC epsilon, PKC delta, PKC beta, PKC alpha, Phospho-PRKCA (T197), KPCA_HUMAN

Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKCA (Thr197). Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody can be used in IF,IHC,WB.

Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody

Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody

😃Good
Catalog No. TMAC-03308Alias Protein kinase C zeta, Protein kinase C gamma, Protein kinase C epsilon, Protein kinase C delta, Protein kinase C beta, Protein kinase C alpha, Protein kinase C, PRKCZ, PRKCG, PRKCE, PRKCD, PRKCB2, PRKCB1, PRKCB, PRKCA (p-Thr197), PRKCA (p-T197), PRKCA, p-PRKCA (Thr197), p-PRKCA (T197), PKCG, PKCE, PKCD, PKCB, PKC-alpha, PKC-A, PKCA, PKC2, PKC zeta, PKC gamma, PKC epsilon, PKC delta, PKC beta, PKC alpha, Phospho-PRKCA (T197), KPCA_HUMAN
Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKCA (Thr197). Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody can be used in IF,IHC,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2177-10 days
100 μL$3167-10 days
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Instruction Manual of Detection Antibody

Product Introduction

Bioactivity
Description
Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKCA (Thr197). Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody can be used in IF,IHC,WB.
SynonymsProtein kinase C zeta, Protein kinase C gamma, Protein kinase C epsilon, Protein kinase C delta, Protein kinase C beta, Protein kinase C alpha, Protein kinase C, PRKCZ, PRKCG, PRKCE, PRKCD, PRKCB2, PRKCB1, PRKCB, PRKCA (p-Thr197), PRKCA (p-T197), PRKCA, p-PRKCA (Thr197), p-PRKCA (T197), PKCG, PKCE, PKCD, PKCB, PKC-alpha, PKC-A, PKCA, PKC2, PKC zeta, PKC gamma, PKC epsilon, PKC delta, PKC beta, PKC alpha, Phospho-PRKCA (T197), KPCA_HUMAN
Ig Type
IgG
Reactivity
Human,Mouse
Verified Activity
1. Western blot analysis of extracts from mouse brain cells using PKA α/β CAT (Phospho-Thr197) Antibody TMAC-03308.The lane on the right is treated with the antigen-specific peptide.
2. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA α/β CAT (Phospho-Thr197) antibody TMAC-03308 (left) or the same antibody preincubated with blocking peptide (right).
3. Immunofluorescence staining of methanol-fixed A549 cells using PKA α/β CAT (Phospho-Thr197) Antibody TMAC-03308.
Application
IF
IHC
WB
Recommended Dose
WB: 1:500-1000; IHC: 1:50-100; IF: 1:100-200
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
AppearanceLiquid
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundPhosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Phospho
Antigen Details
Immunogen
Peptide sequence around phosphorylation site of threonine 197(T-W-T(p)-L-C) derived from Human PKA α/β CAT
Antigen Species
human
Chemical Properties
Molecular WeightActual: 40 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

PRKCA (pThr197)PRKCA (pT197)PRKCA (phospho-Thr197)PRKCA (phospho-T197)pPRKCA (Thr197)pPRKCA (T197)
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