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Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody
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Catalog No. TMAC-03308
Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKCA (Thr197). Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody can be used in WB,IHC,IF.
Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody
Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody
😃Good
Catalog No. TMAC-03308
Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKCA (Thr197). Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody can be used in WB,IHC,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $217 | 7-10 days | |
| 100 μL | $316 | 7-10 days |
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Chemical Properties
| Description | Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKCA (Thr197). Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody can be used in WB,IHC,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-100; IF: 1:100-200 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of threonine 197(T-W-T(p)-L-C) derived from Human PKA α/β CAT |
| Antigen Species | human |
| Molecular Weight | Actual: 40 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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