Anti-Phospho-PPIG (Ser376) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-PPIG (Ser376). Anti-Phospho-PPIG (Ser376) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PPIG (Ser376)) Polyclonal Antibody, Unconjugated (TMAB-11180) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
Testis (Mouse) Lysate at 40 μg
HL60 (Human) Cell Lysate at 30 μg
Primary: Anti-Phospho-PPIG (Ser376) (TMAB-11180) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 89 kD
Observed band size: 110 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human PPIG around the phosphorylation site of Ser376

Human

PPIG

Peptidyl-prolyl cis-trans isomerase G

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.