Anti-Phospho-PP2A alpha (Ser307) Antibody (5Q344) is a Rabbit antibody targeting Phospho-PP2A alpha (Ser307). Anti-Phospho-PP2A alpha (Ser307) Antibody (5Q344) can be used in WB,IHC-P,IHC-Fr,IF,IP.

IgG

5Q344

Human,Mouse,Rat

1. 25 μg total protein per lane of various lysates (see on figure) probed with Phospho-PP2A alpha (Ser307) monoclonal antibody, unconjugated (TMAB-11178) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PP2A alpha (Ser307) Monoclonal Antibody, Unconjugated (TMAB-11178) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PP2A alpha (Ser307) Monoclonal Antibody, Unconjugated (TMAB-11178) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PP2A alpha (Ser307) Monoclonal Antibody, Unconjugated (TMAB-11178) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PP2A alpha (Ser307) Monoclonal Antibody, Unconjugated (TMAB-11178) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PP2A alpha (Ser307) Monoclonal Antibody, Unconjugated (TMAB-11178) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200; IP: 1:20-50

Monoclonal

A synthesized peptide: human PP2A alpha around the phosphorylation site of S307

Human

PPP2CA

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.