Anti-Phospho-PKR (Thr446) Antibody (6I657) is a Rabbit antibody targeting Phospho-PKR (Thr446). Anti-Phospho-PKR (Thr446) Antibody (6I657) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

6I657

Human

1. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PKR (Thr446)) Monoclonal Antibody, Unconjugated (TMAB-11163) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PKR (Thr446)) Monoclonal Antibody, Unconjugated (TMAB-11163) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKR (Thr446) Monoclonal Antibody, Unconjugated (TMAB-11163) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKR (Thr446) Monoclonal Antibody, Unconjugated (TMAB-11163) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Hela (H) cells were treated with or without Calyculin A (100 ng/mL) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-PKR (Thr446) monoclonal antibody, unconjugated (TMAB-11163) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200

Monoclonal

A synthesized peptide: human eIF2A K2 around the phosphorylation site of T446

Human

EIF2AK2

Interferon-induced, double-stranded RNA-activated protein kinase

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.