Anti-Phospho-PKM2 (Tyr148) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PKM2 (Tyr148). Anti-Phospho-PKM2 (Tyr148) Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Pig,Cow,Rabbit,Sheep,GuineaPig)

Blank control: A549.
Primary Antibody (green line): Rabbit Anti-Phospho-PKM2 (Tyr148) antibody (TMAB-11158)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human PKM2 around the phosphorylation site of Tyr148

Human

PKM

Pyruvate kinase PKM

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.