Anti-Phospho-PKA C alpha/beta/gamma (Thr197) Antibody (2L360) is a Rabbit antibody targeting Phospho-PKA C alpha/beta/gamma (Thr197). Anti-Phospho-PKA C alpha/beta/gamma (Thr197) Antibody (2L360) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

2L360

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (TMAB-11145) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (TMAB-11145) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (TMAB-11145) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (TMAB-11145) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (TMAB-11145) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (TMAB-11145) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. 25 μg total protein per lane of various lysates (see on figure) probed with Phospho-PKA C alpha/beta/gamma (T197) monoclonal antibody, unconjugated (TMAB-11145) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200

Monoclonal

A synthesized peptide: human PKA C alpha around the phosphorylation site of T197

Human

PRKACG

cAMP-dependent protein kinase catalytic subunit gamma

Phosphorylates a large number of substrates in the cytoplasm and the nucleus.