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Anti-Phospho-PIM1 (Tyr309) Polyclonal Antibody
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Catalog No. TMAC-03298
Alias p-PIM1 (Y309), p-PIM1 (Tyr309), PIM1 (p-Y309), PIM1 (p-Tyr309)
Anti-Phospho-PIM1 (Tyr309) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PIM1 (Tyr309). Anti-Phospho-PIM1 (Tyr309) Polyclonal Antibody can be used in WB.
Anti-Phospho-PIM1 (Tyr309) Polyclonal Antibody
Anti-Phospho-PIM1 (Tyr309) Polyclonal Antibody
😃Good
Catalog No. TMAC-03298Alias p-PIM1 (Y309), p-PIM1 (Tyr309), PIM1 (p-Y309), PIM1 (p-Tyr309)
Anti-Phospho-PIM1 (Tyr309) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PIM1 (Tyr309). Anti-Phospho-PIM1 (Tyr309) Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $217 | 7-10 days | |
| 100 μL | $318 | 7-10 days |
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Chemical Properties
| Description | Anti-Phospho-PIM1 (Tyr309) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PIM1 (Tyr309). Anti-Phospho-PIM1 (Tyr309) Polyclonal Antibody can be used in WB. |
| Synonyms | p-PIM1 (Y309), p-PIM1 (Tyr309), PIM1 (p-Y309), PIM1 (p-Tyr309) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of extracts from HUVEC cells treated with PMA using Pim-1 (Phospho-Tyr309) Antibody TMAC-03298. The lane on the right is treated with the antigen-specific peptide. |
| Application | |
| Recommended Dose | WB: 1:500-1000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 309(H-R-Y(p)-H-G) derived from Human Pim-1 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 45 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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