Anti-Phospho-PIK3R2 (Tyr467) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PIK3R2 (Tyr467). Anti-Phospho-PIK3R2 (Tyr467) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Dog,Cow,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PIK3R2 (Tyr467)) Polyclonal Antibody, Unconjugated (TMAB-11139) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample: 293T Cell (Human) Lysate at 40 μg
Primary: Anti-phospho-PIK3R2 (Tyr467) (TMAB-11139) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 82 kD
Observed band size: 82 kD
3. Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human A431 cell lysates
Primary: Anti-phospho-PIK3R2 (Tyr467) (TMAB-11139) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 100 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human PIK3R2 around the phosphorylation site of Tyr467

Human

PIK3R2

Phosphatidylinositol 3-kinase regulatory subunit beta

Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.