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Anti-Phospho-PIK3C3 (Ser282) Polyclonal Antibody

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Catalog No. TMAB-11137 Copy Product Info
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Anti-Phospho-PIK3C3 (Ser282) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PIK3C3 (Ser282). Anti-Phospho-PIK3C3 (Ser282) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Phospho-PIK3C3
(Ser282) Polyclonal Antibody

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2207-10 days7-10 days
100 µL$3747-10 days7-10 days
200 µL$5287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-PIK3C3 (Ser282) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PIK3C3 (Ser282). Anti-Phospho-PIK3C3 (Ser282) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-PIK3C3 (Ser282)) Polyclonal Antibody, Unconjugated (TMAB-11137) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-PIK3C3 (Ser282)) Polyclonal Antibody, Unconjugated (TMAB-11137) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMidbody. Late endosome.
Tissue SpecificityUbiquitously expressed, with a highest expression in skeletal muscle.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundPI 3 Kinase Class 3 is a member of the PI3/PI4-kinase family. It is the catalytic subunit of the PI3K complex. It is involved in the transport of lysosomal enzyme precursors to lysosomes. It is ubiquitously expressed, with a highest expression in skeletal muscle.
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human PIK3C3 around the phosphorylation site of Ser282
Antigen Species
Human
Gene Name
PIK3C3
Gene ID
Protein Name
Phosphatidylinositol 3-kinase catalytic subunit type 3
Uniprot ID
Function
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes.
Chemical Properties
Molecular WeightTheoretical: 98 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Anti-Phospho-PIK3C3 (Ser282) Polyclonal Antibody molecular weight