Anti-Phospho-PFKM/PFK1 (Ser775) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PFKM/PFK1 (Ser775). Anti-Phospho-PFKM/PFK1 (Ser775) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Pig,Cow,Horse,Sheep)

1. Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-phospho-PFKL (Ser775) Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 1: Human A431 cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human Raji cell lysates
Lane 4: Human SH-SY5Y cell lysates
Lane 5: Human LOVO cell lysates
Lane 6: Human MCF-7 cell lysates
Primary: Anti-phospho-PFKM/PFK1 (Ser775) (TMAB-11134) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 77 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human PFKM around the phosphorylation site of Ser775

Human

PFKM

ATP-dependent 6-phosphofructokinase, muscle type

Catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP.