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Anti-Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody
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Catalog No. TMAB-11133
Anti-Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PFKFB3/PFK2 (Ser467). Anti-Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody
Anti-Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-11133
Anti-Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PFKFB3/PFK2 (Ser467). Anti-Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PFKFB3/PFK2 (Ser467). Anti-Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Sample: Heart (Mouse) Lysate at 40 μg Primary: Anti-Phospho-PFKFB3/PFK2 (Ser467) (TMAB-11133) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PFKFB3/PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (TMAB-11133) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PFKFB3/PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (TMAB-11133) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PFKFB3 PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (TMAB-11133) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Tissue Specificity | Ubiquitous. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016] |
| Immunogen | KLH conjugated synthesised phosphopeptide: human PFK2 around the phosphorylation site of Ser467 |
| Antigen Species | Human |
| Gene Name | PFKFB3 |
| Gene ID | |
| Protein Name | 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 |
| Uniprot ID | |
| Function | Synthesis and degradation of fructose 2,6-bisphosphate. |
| Molecular Weight | Theoretical: 60 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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