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Anti-Phospho-PERK (Thr980) Polyclonal Antibody

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Catalog No. TMAB-01476
Alias WRS, PRKR-like endoplasmic reticulum kinase, p-PERK (Thr980), p-PERK (T980), PERK (p-Thr980), PERK (p-T980), PEK, Pancreatic eIF2-alpha kinase, Pancreatic eIF2 alpha kinase, HsPEK, HRI, Heme regulated EIF2 alpha kinase, Eukaryotic translation initiation factor 2 alpha kinase 3, EIF2AK3, EC 2.7.11.1, E2AK3, DKFZp781H1925

Anti-Phospho-PERK (Thr980) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PERK (Thr980). Anti-Phospho-PERK (Thr980) Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-Phospho-PERK (Thr980) Polyclonal Antibody

Anti-Phospho-PERK (Thr980) Polyclonal Antibody

😃Good
Catalog No. TMAB-01476Alias WRS, PRKR-like endoplasmic reticulum kinase, p-PERK (Thr980), p-PERK (T980), PERK (p-Thr980), PERK (p-T980), PEK, Pancreatic eIF2-alpha kinase, Pancreatic eIF2 alpha kinase, HsPEK, HRI, Heme regulated EIF2 alpha kinase, Eukaryotic translation initiation factor 2 alpha kinase 3, EIF2AK3, EC 2.7.11.1, E2AK3, DKFZp781H1925
Anti-Phospho-PERK (Thr980) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PERK (Thr980). Anti-Phospho-PERK (Thr980) Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-PERK (Thr980) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PERK (Thr980). Anti-Phospho-PERK (Thr980) Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.
AliasWRS, PRKR-like endoplasmic reticulum kinase, p-PERK (Thr980), p-PERK (T980), PERK (p-Thr980), PERK (p-T980), PEK, Pancreatic eIF2-alpha kinase, Pancreatic eIF2 alpha kinase, HsPEK, HRI, Heme regulated EIF2 alpha kinase, Eukaryotic translation initiation factor 2 alpha kinase 3, EIF2AK3, EC 2.7.11.1, E2AK3, DKFZp781H1925
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Rabbit)
Verified Activity
1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-PERK (Thr980)) polyclonal Antibody, Unconjugated (TMAB-01476) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PERk (Thr980)) Polyclonal Antibody, Unconjugated (TMAB-01476) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Blank control (black line): K562. Primary Antibody (green line): Rabbit Anti-Phospho-PERK (Thr980) antibody (TMAB-01476)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (TMAB-01476) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (TMAB-01476) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (TMAB-01476) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (TMAB-01476) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (TMAB-01476) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (TMAB-01476) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (TMAB-01476) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,ICC/IF,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum membrane; Single-pass type I membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Jan 2010].
Antigen Details
Immunogen
KLH conjugated synthesised phosphopeptide: human PERK around the phosphorylation site of Thr980
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Regulation,Eukaryotic Initiation factors (eIF's),Diabetes,Metabolic disorders,Obesity
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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