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Anti-Phospho-PDPK1 (Tyr373+Tyr376) Polyclonal Antibody
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Catalog No. TMAB-11130
Anti-Phospho-PDPK1 (Tyr373+Tyr376) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PDPK1 (Tyr373+Tyr376). Anti-Phospho-PDPK1 (Tyr373+Tyr376) Polyclonal Antibody can be used in WB.
Anti-Phospho-PDPK1 (Tyr373+Tyr376) Polyclonal Antibody
Anti-Phospho-PDPK1 (Tyr373+Tyr376) Polyclonal Antibody
😃Good
Catalog No. TMAB-11130
Anti-Phospho-PDPK1 (Tyr373+Tyr376) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PDPK1 (Tyr373+Tyr376). Anti-Phospho-PDPK1 (Tyr373+Tyr376) Polyclonal Antibody can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-PDPK1 (Tyr373+Tyr376) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PDPK1 (Tyr373+Tyr376). Anti-Phospho-PDPK1 (Tyr373+Tyr376) Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Chicken,Dog,Pig,Cow) |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. |
| Tissue Specificity | Appears to be expressed ubiquitously. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation it is a potentially important target for metabolic drug research. There are three named isoforms. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human PDPK1 around the phosphorylation site of (Y373+Y376) |
| Antigen Species | Human |
| Gene Name | PDPK1 |
| Gene ID | |
| Protein Name | 3-phosphoinositide-dependent protein kinase 1 |
| Uniprot ID | |
| Function | Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. |
| Molecular Weight | Theoretical: 61 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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