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Anti-Phospho-PDGFRB (Y740) Antibody (1I961)
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Catalog No. TMAC-03115
Alias p-PDGFRB (Y740), PDGFRB (p-Y740)
Anti-Phospho-PDGFRB (Y740) Antibody (1I961) is a Rabbit antibody targeting Phospho-PDGFRB (Y740). Anti-Phospho-PDGFRB (Y740) Antibody (1I961) can be used in ICC/IF,WB.
Anti-Phospho-PDGFRB (Y740) Antibody (1I961)
Anti-Phospho-PDGFRB (Y740) Antibody (1I961)
😃Good
Catalog No. TMAC-03115Alias p-PDGFRB (Y740), PDGFRB (p-Y740)
Anti-Phospho-PDGFRB (Y740) Antibody (1I961) is a Rabbit antibody targeting Phospho-PDGFRB (Y740). Anti-Phospho-PDGFRB (Y740) Antibody (1I961) can be used in ICC/IF,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-Phospho-PDGFRB (Y740) Antibody (1I961) is a Rabbit antibody targeting Phospho-PDGFRB (Y740). Anti-Phospho-PDGFRB (Y740) Antibody (1I961) can be used in ICC/IF,WB. |
| Synonyms | p-PDGFRB (Y740), PDGFRB (p-Y740) |
| Ig Type | IgG |
| Clone | 1I961 |
| Reactivity | Mouse,Rat |
| Verified Activity | 1. ICC staining PDGF Receptor beta(phospho Y740) in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 2. ICC staining PDGF Receptor beta(phospho Y740) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.   |
| Application | |
| Recommended Dose | WB: 1:1000; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Platelet Derived Growth Factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PDGF-AA, PDGF-AB, and PDGF-BB. These three isoforms bind with different affinities to two receptor types, a and b, which are endowed with protein tyrosine kinase domains and undergo either homo- or heterodimerization as a consequence of ligand binding. Ligand stimulation of PDGFR-b leads to autophosphorylation at Tyr-857, which is the major autophosphorylation site, and Tyr-751, which is the major in vitro phosphorylation site. Autophosphorylation of Tyr-751, which lies in the kinase insert region, is required for binding of phosphatidylinositol-3 kinase to the receptor. These auto-phosphorylation events largely contribute to signal transduction through the PDGF receptor. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | A synthesized phosphopeptide: human PDGF Receptor beta around the phosphorylation site of Tyr740 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 190 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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