Anti-Phospho-PDCD4 (Ser457) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-PDCD4 (Ser457). Anti-Phospho-PDCD4 (Ser457) Polyclonal Antibody 2 can be used in WB,ICC/IF.

IgG

Human,Mouse (predicted:Rat,Chicken,Pig,Horse,Rabbit,Zebrafish,Sheep)

1. Sample: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-phospho-PDCD4 (Ser457) (TMAB-11122) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
2. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-PDCD4 (Ser457)) polyclonal Antibody, Unconjugated (TMAB-11122) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; ICC/IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human PDCD4 around the phosphorylation site of Ser457

Human

PDCD4

Programmed cell death protein 4

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.