Anti-Phospho-PBK (Thr9) Antibody (5A495) is a Rabbit antibody targeting Phospho-PBK (Thr9). Anti-Phospho-PBK (Thr9) Antibody (5A495) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

5A495

Human

1. Hela (H) cells were treated with or without Nocodazole, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-PBK (Thr9) monoclonal antibody, unconjugated (TMAB-11121) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PBK (Thr9) Monoclonal Antibody, Unconjugated (TMAB-11121) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PBK (Thr9) Monoclonal Antibody, Unconjugated (TMAB-11121) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-PBK (Thr9) Monoclonal Antibody, Unconjugated (TMAB-11121) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200

Monoclonal

A synthesized peptide: human PBK around the phosphorylation site of T9

Human

PBK

Lymphokine-activated killer T-cell-originated protein kinase

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.