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Anti-Phospho-Paxillin (Ser83) Polyclonal Antibody 2
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Catalog No. TMAB-11114
Anti-Phospho-Paxillin (Ser83) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-Paxillin (Ser83). Anti-Phospho-Paxillin (Ser83) Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-Paxillin (Ser83) Polyclonal Antibody 2
Anti-Phospho-Paxillin (Ser83) Polyclonal Antibody 2
Copy Product Info😃Good
Catalog No. TMAB-11114
Anti-Phospho-Paxillin (Ser83) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-Paxillin (Ser83). Anti-Phospho-Paxillin (Ser83) Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-Paxillin (Ser83) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-Paxillin (Ser83). Anti-Phospho-Paxillin (Ser83) Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Rat (predicted:Mouse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Paxillin (Ser83)) Polyclonal Antibody, Unconjugated (TMAB-11114) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Paxillin (Ser83)) Polyclonal Antibody, Unconjugated (TMAB-11114) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex. Note=Colocalizes with integrins at the cell periphery. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Concentration | 1mg/ml |
| Research Background | Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk). |
| Immunogen | KLH conjugated Synthesised phosphopeptide: rat Paxillin around the phosphorylation site of Ser83 |
| Antigen Species | Rat |
| Gene Name | PXN |
| Gene ID | |
| Protein Name | Paxillin |
| Uniprot ID | |
| Function | Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). |
| Molecular Weight | Theoretical: 68 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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