Anti-Phospho-PAK2 (Ser141) Polyclonal Antibody 3 is a Rabbit antibody targeting Phospho-PAK2 (Ser141). Anti-Phospho-PAK2 (Ser141) Polyclonal Antibody 3 can be used in IHC-P,IHC-Fr,IF.

IgG

Mouse (predicted:Human,Rat,Dog,Cow,Horse,Rabbit)

Tissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-p-PAK1 (Ser141) polyclonal Antibody, Unconjugated (TMAB-11105) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human PAK2 around the phosphorylation site of Ser141

Human

PAK2

Serine/threonine-protein kinase PAK 2

The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.