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Anti-Phospho-p53BP1 (Ser25+Ser29) Polyclonal Antibody
Catalog No. TMAB-11090 Copy Product Info
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Anti-Phospho-p53BP1 (Ser25+Ser29) Polyclonal Antibody is a Rabbit antibody targeting Phospho-p53BP1 (Ser25+Ser29). Anti-Phospho-p53BP1 (Ser25+Ser29) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-p53BP1 (Ser25+Ser29) Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-11090
Anti-Phospho-p53BP1 (Ser25+Ser29) Polyclonal Antibody is a Rabbit antibody targeting Phospho-p53BP1 (Ser25+Ser29). Anti-Phospho-p53BP1 (Ser25+Ser29) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-p53BP1
(Ser25+Ser29) Polyclonal Antibody
(Ser25+Ser29) Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Bioactivity
Antigen Details
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| Description | Anti-Phospho-p53BP1 (Ser25+Ser29) Polyclonal Antibody is a Rabbit antibody targeting Phospho-p53BP1 (Ser25+Ser29). Anti-Phospho-p53BP1 (Ser25+Ser29) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-p53BP1 (Ser25+Ser29)) Polyclonal Antibody, Unconjugated (TMAB-11090) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-p53BP1 (Ser25+Ser29)) Polyclonal Antibody, Unconjugated (TMAB-11090) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Chromosome; centromere; kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | p53 binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, p53 binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double strand breaks. This also suggests a role in DNA repair, maintaining genomic stability. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human p53BP1 around the phosphorylation site of Ser25/29 |
| Antigen Species | Human |
| Gene Name | TP53BP1 |
| Gene ID | |
| Protein Name | TP53-binding protein 1 |
| Uniprot ID | |
| Function | May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. |
| Molecular Weight | Theoretical: 213 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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