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Anti-Phospho-p53 (Ser15) Polyclonal Antibody
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Catalog No. TMAB-01475
Anti-Phospho-p53 (Ser15) Polyclonal Antibody is a Rabbit antibody targeting Phospho-p53 (Ser15). Anti-Phospho-p53 (Ser15) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Anti-Phospho-p53 (Ser15) Polyclonal Antibody
Anti-Phospho-p53 (Ser15) Polyclonal Antibody
😃Good
Catalog No. TMAB-01475
Anti-Phospho-p53 (Ser15) Polyclonal Antibody is a Rabbit antibody targeting Phospho-p53 (Ser15). Anti-Phospho-p53 (Ser15) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-p53 (Ser15) Polyclonal Antibody is a Rabbit antibody targeting Phospho-p53 (Ser15). Anti-Phospho-p53 (Ser15) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Dog,Pig,Cow,Rabbit,Sheep,GuineaPig) |
| Verified Activity | 1. Tissue/cell: Mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-p53 Polyclonal Antibody, Unconjugated (TMAB-01475) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Blank control (blue line): MCF 7 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-P53 (Ser15) antibody (TMAB-01475), Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg/test. 3. A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-P53 (Ser15)) polyclonal Antibody, Unconjugated (TMAB-01475) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 4. Sample: Lane 1: Human A549 cell lysates Lane 2: Human MCF-7 cell lysates Primary: Anti-phospho-P53 (Ser15) (TMAB-01475) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 53 kDa |
| Application | WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1μg /test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Note=Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2. Isoform 1: Nucleus. Cytoplasm. Note=Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4. Isoform 2: Nucleus. Cytoplasm. Note=Localized mainly in the nucleus with minor staining in the cytoplasm. Isoform 3: Nucleus. Cytoplasm. Note=Localized in the nucleus in most cells but found in the cytoplasm in some cells. Isoform 4: Nucleus. Cytoplasm. Note=Predominantly nuclear but translocates to the cytoplasm following cell stress. Isoform 7: Nucleus. Cytoplasm. Note=Localized mainly in the nucleus with minor staining in the cytoplasm. Isoform 8: Nucleus. Cytoplasm. Note=Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli. Isoform 9: Cytoplasm. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human P53 around the phosphorylation site of Ser15 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | p53 pathway,p53 pathway,p53 Pathway,p53,p53,p53,Tumor Suppressors |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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