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Anti-Phospho-Nucleolar transcription factor 1 (Ser484) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Nucleolar transcription factor 1 (Ser484). Anti-Phospho-Nucleolar transcription factor 1 (Ser484) Polyclonal Antibody can be used in IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $216 | 7-10 days | 7-10 days | |
| 100 μL | $316 | 7-10 days | 7-10 days |
| Description | Anti-Phospho-Nucleolar transcription factor 1 (Ser484) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Nucleolar transcription factor 1 (Ser484). Anti-Phospho-Nucleolar transcription factor 1 (Ser484) Polyclonal Antibody can be used in IHC,WB. |
| Synonyms | p-Nucleolar transcription factor 1 (Ser484), p-Nucleolar transcription factor 1 (S484), Nucleolar transcription factor 1 (p-Ser484), Nucleolar transcription factor 1 (p-S484) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using UBF (Phospho-Ser484) antibody TMAC-04188. The lane on the right is treated with the synthesized peptide. 2. Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using UBF (Phospho-Ser484) antibody TMAC-04188. The picture on the right is treated with the synthesized peptide. ![]() ![]() |
| Application | |
| Recommended Dose | WB: 1:500-3000; IHC: 1:50-100 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of serine 484 (P-E-S(p)-P-K) derived from Human UBF |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 85 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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