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Anti-Phospho-NFKBIA (S32) Antibody (6R546) is a Rabbit antibody targeting Phospho-NFKBIA (S32). Anti-Phospho-NFKBIA (S32) Antibody (6R546) can be used in WB,ICC,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $497 | 7-10 days |
Description | Anti-Phospho-NFKBIA (Ser32) Antibody (6R546) is a Rabbit antibody targeting Phospho-NFKBIA (Ser32). Anti-Phospho-NFKBIA (Ser32) Antibody (6R546) can be used in WB,ICC,IP. |
Synonyms | p-NFKBIA (Ser32), p-NFKBIA (S32), NFKBIA (p-Ser32), NFKBIA (p-S32) |
Ig Type | IgG |
Clone | 6R546 |
Reactivity | Human |
Verified Activity | 1. Western blot analysis of Phospho-IKB alpha (S32) on Ags cell lysates using anti-Phospho-IKB alpha (S32) antibody at 1/1,000 dilution. 2. ICC staining Phospho-IKB alpha (S32) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Phospho-IKB alpha (S32) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:1000; ICC: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IκB family is represented by a protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IκB family member, IκB-ε, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel. |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | A synthesized phosphopeptide: human IKB alpha around the phosphorylation site of Ser32 |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Theoretical: 36 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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