Anti-Phospho-NFATc2 (Ser330) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NFATc2 (Ser330). Anti-Phospho-NFATc2 (Ser330) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse)

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (phospho-NFATc2 (Ser330)) Polyclonal Antibody, Unconjugated (TMAB-11040) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
2. Blank control (black line): Molt4.
Primary Antibody (green line): Rabbit Anti-phospho-NFATc2 (Ser330) antibody (TMAB-11040)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
3. Sample:
Lane 1: Mouse Thymus tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Human Raji cell lysates
Lane 4: Human Jurkat cell lysates
Lane 5: Human U251 cell lysates
Primary: Anti-phospho-NFATc2 (Ser330) (TMAB-11040) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 140 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human NFATc2 around the phosphorylation site of Ser330

Human

NFATC2

Nuclear factor of activated T-cells, cytoplasmic 2

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.