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Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody
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Catalog No. TMAB-01467
Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NF-kB p65 (Ser536). Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody
Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody
😃Good
Catalog No. TMAB-01467
Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NF-kB p65 (Ser536). Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NF-kB p65 (Ser536). Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Monkey) |
| Verified Activity | NIH/3T3 (M) cells were treated with or without Calyculin A (100nM) for 30 min, 25 μg total protein per Lane of cell lysates probed with phospho-NFKB p65 polyclonal antibody, unconjugated (TMAB-01467) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm. Note=Colocalized with DDX1 in the nucleus upon TNF-alpha induction. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human NFKBp65 around the phosphorylation site of Ser536 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | RelA (p65),NFkB pathway,Inflammatory mediators,RelA (p65),NFkB pathway,Obesity,Host Immune Response,Host Virus Interaction,Alzheimer's disease,NFkB Pathway |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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