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Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody

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Catalog No. TMAB-01467
Alias p-NF-κB p65 (Ser536), p-NF-κB p65 (S536), Phospho-NF-κB p65 (S536), p65, NF-κB p65 (p-Ser536), NF-κB p65 (p-S536), NF-κB, NFκB, NF-κ B, NFKB3, NF-kB, NFkB, NF-kappaB, NFkappaB, NF-kappa B, NF-k B, CMCU, AIF3BL3

Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NF-kB p65 (Ser536). Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody

Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody

😃Good
Catalog No. TMAB-01467Alias p-NF-κB p65 (Ser536), p-NF-κB p65 (S536), Phospho-NF-κB p65 (S536), p65, NF-κB p65 (p-Ser536), NF-κB p65 (p-S536), NF-κB, NFκB, NF-κ B, NFKB3, NF-kB, NFkB, NF-kappaB, NFkappaB, NF-kappa B, NF-k B, CMCU, AIF3BL3
Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NF-kB p65 (Ser536). Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Instruction Manual of Detection Antibody

Product Introduction

Bioactivity
Description
Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NF-kB p65 (Ser536). Anti-Phospho-NF-kB p65 (Ser536) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Aliasp-NF-κB p65 (Ser536), p-NF-κB p65 (S536), Phospho-NF-κB p65 (S536), p65, NF-κB p65 (p-Ser536), NF-κB p65 (p-S536), NF-κB, NFκB, NF-κ B, NFKB3, NF-kB, NFkB, NF-kappaB, NFkappaB, NF-kappa B, NF-k B, CMCU, AIF3BL3
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat,Monkey)
Verified Activity
NIH/3T3 (M) cells were treated with or without Calyculin A (100nM) for 30 min, 25 μg total protein per Lane of cell lysates probed with phospho-NFKB p65 polyclonal antibody, unconjugated (TMAB-01467) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
Application
WB
IHC-P
IHC-Fr
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Cytoplasm. Note=Colocalized with DDX1 in the nucleus upon TNF-alpha induction. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundNF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human NFKBp65 around the phosphorylation site of Ser536
Antigen Species
Human
Gene ID
5970
Uniprot ID
Q04206
Biology Area
RelA (p65),NFkB pathway,Inflammatory mediators,RelA (p65),NFkB pathway,Obesity,Host Immune Response,Host Virus Interaction,Alzheimer's disease,NFkB Pathway
Chemical Properties
Molecular WeightTheoretical: 61 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

pNF-κB p65 (Ser536)pNF-κB p65 (S536)NF-κB p65 (pSer536)NF-κB p65 (pS536)NF-κB p65 (phospho-Ser536)NF-κB p65 (phospho-S536)
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