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Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703)
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Catalog No. TMAY-01674
Alias p65, NF-κB, NFκB, NF-κ B, NFKB3, NF-kB, NFkB, NF-kappaB, NFkappaB, NF-kappa B, NF-k B, CMCU, AIF3BL3
Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) is a Rabbit antibody targeting Phospho-NF-κB p65 (Ser536). Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) can be used in WB.
Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703)
Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703)
😃Good
Catalog No. TMAY-01674Alias p65, NF-κB, NFκB, NF-κ B, NFKB3, NF-kB, NFkB, NF-kappaB, NFkappaB, NF-kappa B, NF-k B, CMCU, AIF3BL3
Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) is a Rabbit antibody targeting Phospho-NF-κB p65 (Ser536). Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $81 | 7-10 days | |
| 100 μL | $235 | 7-10 days |
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Chemical Properties
| Description | Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) is a Rabbit antibody targeting Phospho-NF-κB p65 (Ser536). Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) can be used in WB. |
| Alias | p65, NF-κB, NFκB, NF-κ B, NFKB3, NF-kB, NFkB, NF-kappaB, NFkappaB, NF-kappa B, NF-k B, CMCU, AIF3BL3 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 5Y703 |
| Reactivity | Human;
Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus |
| Verified Activity | 1. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (200 nM, 30 min) (line B); treated with Calyculin A and λ-phosphatase (line C) using Phospho-NF-κB p65 (Ser536) Antibody, Rabbit MAb at 1:1000 dilution. 2. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-NF-κB p65 (Ser536) Antibody, Rabbit MAb at 1:5000 dilution (upper) or Beta-Tubulin Loading Control Antibody, Mouse MAb at 1:20000 dilution (lower). 3. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (200 nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-NF-κB p65 (Ser536) Antibody, Rabbit MAb at 1:5000 dilution. (Validation Experiment) 4. Western blot analysis of extracts from serum-starved NIH/3T3, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-NF-κB p65 (Ser536) Antibody, Rabbit MAb at 1:1000 dilution. (Validation Experiment) |
| Application | WB |
| Recommended Dose | WB: 1:2000-1:20000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Ser536 of the Human NF-κB p65. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Research Background | RELA (v-rel reticuloendotheliosis viral oncogene homolog A), also known as Nuclear factor NF-kappa-B p65 subunit, or Transcription factor p65, is a transcription factor expressed in growth plate chondrocytes where it facilitates chondrogenesis. The v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA) gene encodes the major component of the NF-?B complex. NF-kappaB is a generic name for an evolutionarily conserved transcription-factor system that contributes to the mounting of an effective immune response but is also involved in the regulation of cell proliferation, development, and apoptosis. The implication of NF-kappaB in central biological processes and its extraordinary connectivity to other signaling pathways raise a need for highly controlled regulation of NF-kappaB activity at several levels. The mammalian Rel/NF-kappaB family of transcription factors, including RelA, c-Rel, RelB, NF-kappaB1 (p50 and its precursor p105), and NF-kappaB2 (p52 and its precursor p100), plays a central role in the immune system by regulating several processes ranging from the development and survival of lymphocytes and lymphoid organs to the control of immune responses and malignant transformation. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic phosphopeptide corresponding to residues around Ser536 of the Human NF-κB p65 |
| Antigen Species | Human |
| Biology Area | Apoptosis Transcription Factors and Regulators |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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