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Anti-Phospho-NEUROD1 (Ser274) Polyclonal Antibody 2
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Catalog No. TMAB-11035
Anti-Phospho-NEUROD1 (Ser274) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-NEUROD1 (Ser274). Anti-Phospho-NEUROD1 (Ser274) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-NEUROD1 (Ser274) Polyclonal Antibody 2
Anti-Phospho-NEUROD1 (Ser274) Polyclonal Antibody 2
Copy Product Info😃Good
Catalog No. TMAB-11035
Anti-Phospho-NEUROD1 (Ser274) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-NEUROD1 (Ser274). Anti-Phospho-NEUROD1 (Ser274) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-NEUROD1 (Ser274) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-NEUROD1 (Ser274). Anti-Phospho-NEUROD1 (Ser274) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Pig,Cow,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NeuroD1 (Ser274)) Polyclonal Antibody, Unconjugated (TMAB-11035) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NeuroD1 (Ser274)) Polyclonal Antibody, Unconjugated (TMAB-11035) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NeuroD1 (Ser274)) Polyclonal Antibody, Unconjugated (TMAB-11035) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Sample: Lane 1: Cerebrum (Rat) Lysate at 40 μg Lane 2: Cerebellum (Mouse) Lysate at 40 μg Primary: Anti-phospho-NeuroD1 (Ser274) (TMAB-11035) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated Synthesised acetylpeptide: human NEUROD1 around the acetylation site of Ser274 |
| Antigen Species | Human |
| Gene Name | NEUROD1 |
| Gene ID | |
| Protein Name | Neurogenic differentiation factor 1 |
| Uniprot ID | |
| Function | Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box. |
| Molecular Weight | Theoretical: 40 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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