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Anti-Phospho-NCF1 (Ser345) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NCF1 (Ser345). Anti-Phospho-NCF1 (Ser345) Polyclonal Antibody can be used in IHC,WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $218 | 7-10 days | |
100 μL | $317 | 7-10 days |
Description | Anti-Phospho-NCF1 (Ser345) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NCF1 (Ser345). Anti-Phospho-NCF1 (Ser345) Polyclonal Antibody can be used in IHC,WB. |
Synonyms | p-NCF1 (Ser345), p-NCF1 (S345), NCF1 (p-Ser345), NCF1 (p-S345) |
Ig Type | IgG |
Reactivity | Human |
Verified Activity | 1. Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody TMAC-02968. The lane on the right is treated with the antigen-specific peptide. 2. Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody TMAC-02968 (left) or the same antibody preincubated with blocking peptide (right). |
Application | |
Recommended Dose | WB: 1:500-1000; IHC: 1:50-100 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Appearance | Liquid |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | Peptide sequence around phosphorylation site of Serine 345(P-Q-S(p)-P-G) derived from Human Nuclear p47 phox |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Actual: 45 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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