Anti-Phospho-MYPT1 (Thr696) Polyclonal Antibody 3 is a Rabbit antibody targeting Phospho-MYPT1 (Thr696). Anti-Phospho-MYPT1 (Thr696) Polyclonal Antibody 3 can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Horse,Rabbit)

1. Blank control (blue): Hela cells (fixed with 2% paraformaldehyde (10 min),then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-Phospho-MYPT1 (Thr696) antibody (TMAB-11024), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions);
Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
2. Sample: 293T (Human) Cell Lysate at 40 μg
Primary: Anti-p-MYPT1 (Thr696) (TMAB-11024) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 113 kD
Observed band size: 120 kD
3. Sample: BRL-3A (Rat) Cell Lysate at 40 μg
Primary: Anti-p-MYPT1 (Thr696) (TMAB-11024) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 113 kD
Observed band size: 120 kD
4. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human MCF-7 cell lysates
Primary: Anti-Phospho-MYPT1 (Thr696) (TMAB-11024) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 113 kDa
Observed band size: 125 kDa

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthesised phosphopeptide: human MYPT1 around the phosphorylation site of Thr696

Human

PPP1R12A

Protein phosphatase 1 regulatory subunit 12A

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.